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Increased hypothalamic-pituitary-adrenal axis activity and hepatic insulin resistance in low-birth-weight rats


By JPGRAY - Posted on 24 February 2009

TitleIncreased hypothalamic-pituitary-adrenal axis activity and hepatic insulin resistance in low-birth-weight rats
Publication TypeJournal Article
Year of Publication2007
AuthorsBuhl ES, Neschen S, Yonemitsu S, Rossbacher J, Zhang D, Morino K, Flyvbjerg A, Perret P, Samuel V, Kim J, Cline GW, Petersen KF
JournalAm J Physiol Endocrinol Metab
Volume293
Issue5
PaginationE1451-8
Date PublishedNov
Publication Languageeng
ISBN Number0193-1849 (Print)
Accession Number17895287
Key WordsReceptors, Rats, Insulin/blood, Female, Animals, Newborn, Triglycerides/blood, Reverse Transcriptase Polymerase Chain Reaction, Restraint, Physical/physiology, Glucocorticoid/genetics/metabolism, Sprague-Dawley, Random Allocation, Pregnancy, Pituitary-Adrenal System/*metabolism, Phosphoenolpyruvate Carboxykinase (ATP)/genetics/metabolism, Liver/enzymology/*metabolism, Insulin-Like Growth Factor I/genetics/metabolism, Insulin-Like Growth Factor Binding Protein 1/genetics/metabolism, Insulin Resistance/*physiology, Hypothalamo-Hypophyseal System/*metabolism, Glucose/metabolism, Fasting/blood, Corticosterone/blood/urine, Cholesterol/blood, Adrenocorticotropic Hormone/blood
Abstract

Individuals born with a low birth weight (LBW) have an increased prevalence of type 2 diabetes, but the mechanisms responsible for this association are unknown. Given the important role of insulin resistance in the pathogenesis of type 2 diabetes, we examined insulin sensitivity in a rat model of LBW due to intrauterine fetal stress. During the last 7 days of gestation, rat dams were treated with dexamethasone and insulin sensitivity was assessed in the LBW offspring by a hyperinsulinemic euglycemic clamp. The LBW group had liver-specific insulin resistance associated with increased levels of PEPCK expression. These changes were associated with pituitary hyperplasia of the ACTH-secreting cells, increased morning plasma ACTH concentrations, elevated corticosterone secretion during restraint stress, and an approximately 70% increase in 24-h urine corticosterone excretion. These data support the hypothesis that prenatal stress can result in chronic hyperactivity of the hypothalamic-pituitary-adrenal axis, resulting in increased plasma corticosterone concentrations, upregulation of hepatic gluconeogenesis, and hepatic insulin resistance.

Notes

P01 DK068229/DK/NIDDK NIH HHS/United StatesP30 DK45735/DK/NIDDK NIH HHS/United StatesR01 AG23686/AG/NIA NIH HHS/United StatesR01 DK40936/DK/NIDDK NIH HHS/United StatesJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tUnited States

URLhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17895287
Citation Key307
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