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You are hereRecent Publications of Members of the Boston Ithaca Islet Club / Simultaneous optical measurements of cytosolic Ca2+ and cAMP in single cells

Simultaneous optical measurements of cytosolic Ca2+ and cAMP in single cells

By JPGRAY - Posted on 24 February 2009

Title	Simultaneous optical measurements of cytosolic Ca2+ and cAMP in single cells
Publication Type	Journal Article
Year of Publication	2006
Authors	Harbeck MC, Chepurny O, Nikolaev VO, Lohse MJ, Holz GG, Roe MW
Journal	Sci STKE
Volume	2006
Issue	353
Pagination	pl6
Date Published	Sep 19
Publication Language	eng
ISBN Number	1525-8882 (Electronic)
Accession Number	16985238
Key Words	Transfection, Reproducibility of Results, Mice, Animals, Cell Line, Tumor, Second Messenger Systems, Recombinant Proteins/biosynthesis, Calcium/analysis, Biosensing Techniques, Guanine Nucleotide Exchange Factors/genetics, Fura-2, Fluorescent Dyes, Fluorescence Resonance Energy Transfer/methods, Cytosol/chemistry, Cyclic AMP/analysis, Cations, Divalent
Abstract	Understanding the temporal and spatial integration of the Ca2+ and adenosine 3',5'-monophosphate (cAMP) signaling pathways requires concurrent measurements of both second messengers. Here, we describe an optical technique to simultaneously image cAMP and Ca2+ concentration gradients in MIN6 mouse insulinoma cells using Epac1-camps, a Forster (or fluorescence) resonance energy transfer (FRET)-based cAMP biosensor, and Fura-2, a fluorescent indicator of Ca2+. This real-time imaging method allows investigation of the dynamic organization and integration of multiple levels of signal processing in single living cells.
Notes	Journal ArticleUnited Statessignal transduction knowledge environment
URL	http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16985238
Citation Key	359
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