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Effects of cellular ATP depletion on glucose transport and insulin signaling in 3T3-L1 adipocytes

By JPGRAY - Posted on 24 February 2009

TitleEffects of cellular ATP depletion on glucose transport and insulin signaling in 3T3-L1 adipocytes
Publication TypeJournal Article
Year of Publication2001
AuthorsKang J, Heart E, Sung CK
JournalAm J Physiol Endocrinol Metab
Volume280
Issue3
PaginationE428-35
Date PublishedMar
Publication Languageeng
ISBN Number0193-1849 (Print)
Accession Number11171597
Key WordsMonosaccharide Transport Proteins/metabolism, Mice, *Insulin Resistance, Insulin/*metabolism, Glucose Transporter Type 1, Glucose/*metabolism, Cell Membrane/metabolism, Animals, Adipocytes/drug effects/*metabolism, Adenosine Triphosphate/*metabolism, 3T3 Cells, Sodium Azide/pharmacology, *Signal Transduction, Oxidative Phosphorylation/drug effects, Mitochondria/metabolism, Hexokinase/metabolism, Guanosine/analogs & derivatives/metabolism, Glucosamine/pharmacology, Electron Transport/drug effects, Dinitrophenols/pharmacology, Biological Transport/drug effects
Abstract

Glucosamine induced insulin resistance in 3T3-L1 adipocytes, which was associated with a 15% decrease in cellular ATP content. To study the role of ATP depletion in insulin resistance, we employed sodium azide (NaN3) and dinitrophenol (DNP), which affect mitochondrial oxidative phosphorylation, to achieve a similar 15% ATP depletion. Unlike glucosamine, NaN3 and DNP markedly increased basal glucose transport, and the increased basal glucose transport was associated with increased GLUT-1 content in the plasma membrane without changes in total GLUT-1 content. These agents, like glucosamine, did not affect the early insulin signaling that is implicated in insulin stimulation of glucose transport. In cells with a severe 40% ATP depletion, basal glucose transport was similarly elevated, and insulin-stimulated glucose transport was similar in cells with 15% ATP depletion. In these cells, however, early insulin signaling was severely diminished. These data suggest that cellular ATP depletion by glucosamine, NaN3, and DNP exerts differential effects on basal and insulin-stimulated glucose transport and that ATP depletion per se does not induce insulin resistance in 3T3-L1 adipocytes.
Notes

DK-51015/DK/NIDDK NIH HHS/United StatesComparative StudyJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.United States
URLhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11171597
Citation Key396
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