You are hereRecent Publications of Members of the Boston Ithaca Islet Club / In vivo multimodal imaging of transplanted pancreatic islets
In vivo multimodal imaging of transplanted pancreatic islets
| Title | In vivo multimodal imaging of transplanted pancreatic islets |
| Publication Type | Journal Article |
| Year of Publication | 2006 |
| Authors | |
| Journal | Nat Protoc |
| Volume | 1 |
| Issue | 1 |
| Pagination | 429-35 |
| Publication Language | eng |
| ISBN Number | 1750-2799 (Electronic) |
| Accession Number | 17406265 |
| Key Words | Mice, Magnetic Resonance Imaging/*methods, *Islets of Langerhans Transplantation, Islets of Langerhans/*cytology, Humans, Fluorescent Dyes, Animals, Staining and Labeling/*methods, Nanoparticles/chemistry, Molecular Probe Techniques |
| Abstract | Interest is increasing in the transplantation of pancreatic islets as a means to achieve insulin independence in individuals with type I diabetes. The success of this approach is hampered by the absence of methods to follow the fate of transplanted islets non-invasively. In vivo imaging seems to be the most appropriate technique to achieve this goal in small animals and eventually in humans. Here we describe a protocol for labeling and subsequent imaging of transplanted islets in vivo using magnetic resonance imaging (MRI) and optical imaging. The whole series of experiments can be carried out in roughly 48 h. We believe that our approach can significantly advance the current ability to determine islet distribution, and possibly survival, after transplantation. This information would be essential not only for the long-term monitoring of graft function but also for the design of improved transplantation and immunomodulatory methods. |
| Notes | DK071225/DK/NIDDK NIH HHS/United StatesDK072137/DK/NIDDK NIH HHS/United StatesJournal ArticleResearch Support, N.I.H., ExtramuralEngland |
| URL | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17406265 |
| Citation Key | 443 |
| Export |