You are hereRecent Publications of Members of the Boston Ithaca Islet Club / TRB3 links the E3 ubiquitin ligase COP1 to lipid metabolism

TRB3 links the E3 ubiquitin ligase COP1 to lipid metabolism


By JPGRAY - Posted on 24 February 2009

TitleTRB3 links the E3 ubiquitin ligase COP1 to lipid metabolism
Publication TypeJournal Article
Year of Publication2006
AuthorsQi L, Heredia JE, Altarejos JY, Screaton R, Goebel N, Niessen S, Macleod IX, Liew CW, Kulkarni RN, Bain J, Newgard C, Nelson M, Evans RM, Yates J, Montminy M
JournalScience
Volume312
Issue5781
Pagination1763-6
Date PublishedJun 23
Publication Languageeng
ISBN Number1095-9203 (Electronic)
Accession Number16794074
Key WordsEnergy Metabolism, Dietary Fats/administration & dosage, Cell Line, Cell Cycle Proteins/*metabolism, Animals, Adipose Tissue, 3T3-L1 Cells, Weight Gain, Phosphorylation, Oxidation-Reduction, Mice, Transgenic, Lipolysis, *Lipid Metabolism, Humans, Gene Expression, Fatty Acids/metabolism, Fasting, Brown/cytology/metabolism, Adipose Tissue/cytology/*metabolism, Adipocytes/metabolism, Acetyl-CoA Carboxylase/antagonists & inhibitors/*metabolism, Ubiquitin-Protein Ligases/*metabolism, Ubiquitin/metabolism, Thinness, Obesity/prevention & control, Nuclear Proteins/*metabolism
Abstract

During fasting, increased concentrations of circulating catecholamines promote the mobilization of lipid stores from adipose tissue in part by phosphorylating and inactivating acetyl-coenzyme A carboxylase (ACC), the rate-limiting enzyme in fatty acid synthesis. Here, we describe a parallel pathway, in which the pseudokinase Tribbles 3 (TRB3), whose abundance is increased during fasting, stimulates lipolysis by triggering the degradation of ACC in adipose tissue. TRB3 promoted ACC ubiquitination through an association with the E3 ubiquitin ligase constitutive photomorphogenic protein 1 (COP1). Indeed, adipocytes deficient in TRB3 accumulated larger amounts of ACC protein than did wild-type cells. Because transgenic mice expressing TRB3 in adipose tissue are protected from diet-induced obesity due to enhanced fatty acid oxidation, these results demonstrate how phosphorylation and ubiquitination pathways converge on a key regulator of lipid metabolism to maintain energy homeostasis.

Notes

DK064142/DK/NIDDK NIH HHS/United StatesJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tUnited States

URLhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16794074
Citation Key497
Export

Signup to receive email notifications of upcoming events

To signup to receive email notifications of upcoming BIIC events, fill out the information here.