You are hereRecent Publications of Members of the Boston Ithaca Islet Club / The ATP/DNA ratio is a better indicator of islet cell viability than the ADP/ATP ratio
The ATP/DNA ratio is a better indicator of islet cell viability than the ADP/ATP ratio
| Title | The ATP/DNA ratio is a better indicator of islet cell viability than the ADP/ATP ratio |
| Publication Type | Journal Article |
| Year of Publication | 2008 |
| Authors | |
| Journal | Transplant Proc |
| Volume | 40 |
| Issue | 2 |
| Pagination | 346-50 |
| Date Published | Mar |
| Publication Language | eng |
| ISBN Number | 0041-1345 (Print) |
| Accession Number | 18374063 |
| Key Words | Spectrometry, Reproducibility of Results, Islets of Langerhans/*cytology, Humans, Cell Survival/*physiology, Cell Culture Techniques/methods, Adenosine Triphosphate/*metabolism, Mass, Electrospray Ionization, Insulin/analysis/genetics, Hot Temperature, DNA/*metabolism, Adenosine Diphosphate/*metabolism |
| Abstract | Real-time, accurate assessment of islet viability is critical for avoiding transplantation of nontherapeutic preparations. Measurements of the intracellular ADP/ATP ratio have been recently proposed as useful prospective estimates of islet cell viability and potency. However, dead cells may be rapidly depleted of both ATP and ADP, which would render the ratio incapable of accounting for dead cells. Since the DNA of dead cells is expected to remain stable over prolonged periods of time (days), we hypothesized that use of the ATP/DNA ratio would take into account dead cells and may be a better indicator of islet cell viability than the ADP/ATP ratio. We tested this hypothesis using mixtures of healthy and lethally heat-treated (HT) rat insulinoma cells and human islets. Measurements of ATP/DNA and ADP/ATP from the known mixtures of healthy and HT cells and islets were used to evaluate how well these parameters correlated with viability. The results indicated that ATP and ADP were rapidly (within 1 hour) depleted in HT cells. The fraction of HT cells in a mixture correlated linearly with the ATP/DNA ratio, whereas the ADP/ADP ratio was highly scattered, remaining effectively unchanged. Despite similar limitations in both ADP/ADP and ATP/DNA ratios, in that ATP levels may fluctuate significantly and reversibly with metabolic stress, the results indicated that ATP/DNA was a better measure of islet viability than the ADP/ATP ratio. |
| Notes | U42 RR016598/RR/NCRR NIH HHS/United StatesJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tUnited States |
| URL | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18374063 |
| Citation Key | 512 |
| Export |